ABSTRACT
Low magnesium status has been shown to have a negative impact on glucose homeostasis and insulin sensitivity, as well as on the evolution of diabetic complications. In the present study, 45- to 65- year old type 2 diabetic patients and normal age-matched subjects (n = 40 each) were recruited and their intracellular total magnesium level, serum total magnesium level, and 24-hour urinary total magnesium excretion were assessed. The magnesium levels were measured by spectrophotometric method using calmagite. In type 2 diabetic patients, intracellular magnesium concentration was significantly lower (4.26 ± 0.61 vs. 5.76 ± 0.59 mg/dL, P < 0.001) and 24-hour urinary magnesium concentration significantly higher (125.60 ± 33.32 vs. 72.04 ± 14.55 mg/24 hr, P < 0.001) compared with those of healthy subjects. Serum magnesium levels of diabetic patients and healthy subjects showed no significant difference (1.69 ± 0.30 vs. 1.76 ± 0.31 mg/dL, P > 0.05). There was a significant negative correlation between intracellular and 24-hour urinary magnesium excretion (r = -0.548, n = 80, P < 0.01). There was no correlation between serum and intracellular magnesium (r = -0.021, n = 80, P > 0.05) and, serum and 24 hour urinary magnesium excretion (r = -0.128, n = 80, P > 0.05).
Subject(s)
Diabetes Mellitus, Type 2 , MagnesiumABSTRACT
The main objective of the study is to determine the serum gamma-glutamyl transferase (GGT) and ferritin levels in normotensive and hypertensive monks and to fi nd out whether blood pressure is correlated with serum GGT and ferritin levels in these groups. Normotensive and hypertensive monks age between 40 and 60 years (n=50 each) had participated in the study. Serum GGT was measured by kinetic colorimetric method and serum ferritin level by enzyme-linked immunosorbent assay. The mean serum GGT level of the hypertensive monks was found to be signifi cantly higher than that of the normotensive monks (60.42 ± 25.93 versus 25.32 ± 5.30 U/L) (p < 0.001). The mean serum ferritin level of the hypertensive monks was also found to be signifi cantly higher than that of the normotensive monks (116.87 ± 46.68 versus 37.03 ± 18.79 ng/ml) (p < 0.001). A significant positive correlation between blood pressure (systolic blood pressure, diastolic blood pressure and mean arterial pressure) and serum GGT level was found in hypertensive monks (r = 0.691, p < 0.001; r = 0.482, p < 0.001; r = 0.610, p < 0.001 respectively). No signifi cant correlation between blood pressure and serum ferritin level was found in both groups. An increase in both serum GGT and ferritin levels in the hypertensive group suggested that oxidative stress is involved in the pathophysiology of essential hypertension, and they might rather act as prooxidants in hypertension.
Subject(s)
Hypertension , Vascular ResistanceABSTRACT
To compare the invasive and non-invasive tests for gastric acid secretion, 30 appearently healthy Myanmar male volunteers (25-45 years) were studied. After overnight fasting, they ingested 150 mg of Mg++ metal suspended in 3 mlof glucose and 100 ml of 7
ethanol on the first day and alcohol without Mg++ onthe second day. Basal gastric juice (by Ryle's tube), urine and breath H2 were collected. Another 11 samples of gastric juice and breath H2 were collected aftermeal for 180 minutes at 15 minute intervals. Hourly urine collection was also done for three hours. Acid contents of gastric juice aand urine were determined byconventional titraton method. Breath H2 was analyzed by microanalyzer (Quintron,Model 12 microlyzer). Correlation coefficients: between maximal acid output (15-60 minutes) and 2nd hour urine acid output-0.50(p<0.05); peak acid output (15-60minutes),0.44(p<0.05); and maximal acid output and breath hydrogen test (75-90 minutes), 0.40 (p<0.05). Thus non-invasive tests (Oral Magnesium Breath Hydrogen Test and urine acid output determination) may also be used as alternative tests in studying the gastric acid secretion.